کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755261 | 1050370 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of Notch1 promotes development of human CD8+ single positive T cells in humanized mice
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کلمات کلیدی
HSCmAbeGFPNotch1Monoclonal antibody - آنتی بادی مونوکلونالNOG - با این حالT-ALL - بلند قدT cell development - توسعه سلول Tsingle positive - تک مثبتHematopoietic stem cell - سلول بنیادی هماتوپوئیتT cell acute lymphoblastic leukemia - لوسمی لنفوبلاستی حاد T-سلولیHumanized mouse - ماوس انسانیenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Notch1 mutations are found in more than 50% of human T cell acute lymphoblastic leukemia (T-ALL) cells. However, the functions of Notch1 for human T cell development and leukemogenesis are not well understood. To examine the role of Notch1, human hematopoietic stem cells (HSCs), which had been transduced with a constitutively active form of Notch1 (ICN1), were transplanted into severely immunodeficient NOD/Shi-scid-IL2rγnull (NOG) mice. We found that the great majority of the ICN1-expressing hematopoietic cells in the bone marrow expressed surface markers for T cells, such as CD3, CD4, and CD8, and that this T cell development was independent of the thymus. Accordingly, phenotypically mature CD8+ single positive (SP) T cells were observed in the spleen. Furthermore, T-ALL developed in one NOG recipient mouse out of 26 that had been secondary transferred with the T cells developed in the first NOG mice. These results indicate that Notch1 signaling in HSCs promotes CD8+ SP T cell development, and that T cell leukemogenesis may require additional oncogenic factors other than Notch1 activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 447, Issue 2, 2 May 2014, Pages 346-351
Journal: Biochemical and Biophysical Research Communications - Volume 447, Issue 2, 2 May 2014, Pages 346-351
نویسندگان
Yoichi Haji, Makiko Suzuki, Kunihiko Moriya, Takanori So, Katsuto Hozumi, Masamichi Mizuma, Michiaki Unno, Naoto Ishii,