Expression, purification and substrate specificities of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2

- 3NTDO components were expressed, purified to homogeneity and characterized.
- Reconstituted 3NTDO was in vitro active and removed nitro group efficiently.
- 3NTDO specificity was highest for 3-NT whereas least for 2,4-DNT.
- Methylcatechol formation from 3-NT reveals its degradation pathway in D. sp. DS2.
- Recombinant 3NTDO also yielded chlorocatechols from chloronitro compounds.