کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10756575 | 1050385 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Long-term electrophysiological activity and pharmacological response of a human induced pluripotent stem cell-derived neuron and astrocyte co-culture
ترجمه فارسی عنوان
فعالیت طولانی مدت الکتروفیزیولوژیک و پاسخ فارماکولوژیک از سلول های بنیادی سلول های بنیادی القا شده از سلول های بنیادی و همکاری فرهنگی استروسیت
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کلمات کلیدی
MEAPBShiPSC6-Cyano-7-nitroquinoxaline-2,3-dione - 6-Cyano-7-nitroquinoxaline-2،3-dionemulti-electrode array - آرایه چند الکترودهایPharmacological effects - اثرات فارماکولوژیکLong-term measurement - اندازه گیری درازمدتDIV - دیوdays in vitro - روز in vitroHuman induced pluripotent stem cell - سلول های بنیادی انعقادی انسانی بشرCNQX - سیانکیوایکسPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Human induced pluripotent stem cell (hiPSC)-derived neurons may be effectively used for drug discovery and cell-based therapy. However, the immaturity of cultured human iPSC-derived neurons and the lack of established functional evaluation methods are problematic. We here used a multi-electrode array (MEA) system to investigate the effects of the co-culture of rat astrocytes with hiPSC-derived neurons on the long-term culture, spontaneous firing activity, and drug responsiveness effects. The co-culture facilitated the long-term culture of hiPSC-derived neurons for >3Â months and long-term spontaneous firing activity was also observed. After >3Â months of culture, we observed synchronous burst firing activity due to synapse transmission within neuronal networks. Compared with rat neurons, hiPSC-derived neurons required longer time to mature functionally. Furthermore, addition of the synapse antagonists bicuculline and 6-cyano-7-nitroquinoxaline-2,3-dione induced significant changes in the firing rate. In conclusion, we used a MEA system to demonstrate that the co-culture of hiPSC-derived neurons with rat astrocytes is an effective method for studying the function of human neuronal cells, which could be used for drug screening.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 4, 24 January 2014, Pages 1176-1181
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 4, 24 January 2014, Pages 1176-1181
نویسندگان
A. Odawara, Y. Saitoh, A.H. Alhebshi, M. Gotoh, I. Suzuki,