Temperature-induced labelling of Fluo-3 AM selectively yields brighter nucleus in adherent cells

- We detailedly examine temperature effects of Fluo-3 AM labelling in adherent cells.
- 4 °C Loading and 20 °C de-esterification of Fluo-3 AM yields brighter nuclei.
- Brighter nuclei labelling by Fluo-3 AM also depends on cell adhesion quality.
- A qualitative model of the brighter nucleus is proposed.