کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10757279 | 1050393 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human serum activates CIDEB-mediated lipid droplet enlargement in hepatoma cells
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کلمات کلیدی
PPARPGC-1αADRPVLDLHNF-4αCIDECARS microscopytriglyceride - تریگلیسریدhepatocyte nuclear factor 4α - عامل هسته ای hepatocyte 4αlipid droplet - قطره چربیvery low density lipoprotein - لیپوپروتئین چگالی بسیار کم استCARS - ماشین هاLipid metabolism - متابولیسم لیپیدcoherent anti-Stokes Raman scattering - پراکندگی ضد استوکس رامان منسجمadipose differentiation-related protein - پروتئین مرتبط با تمایز چربیPeroxisome proliferator activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Human serum activates CIDEB-mediated lipid droplet enlargement in hepatoma cells Human serum activates CIDEB-mediated lipid droplet enlargement in hepatoma cells](/preview/png/10757279.png)
چکیده انگلیسی
Human hepatocytes constitutively express the lipid droplet (LD) associated protein cell death-inducing DFFA-like effector B (CIDEB). CIDEB mediates LD fusion, as well as very-low-density lipoprotein (VLDL) maturation. However, there are limited cell culture models readily available to study CIDEB's role in these biological processes, as hepatoma cell lines express negligible levels of CIDEB. Recent work has highlighted the ability of human serum to differentiate hepatoma cells. Herein, we demonstrate that culturing Huh7.5 cells in media supplemented with human serum activates CIDEB expression. This activation occurs through the induced expression of PGC-1α, a positive transcriptional regulator of CIDEB. Coherent anti-Stokes Raman scattering (CARS) microscopy revealed a correlation between CIDEB levels and LD size in human serum treated Huh7.5 cells. Human serum treatment also resulted in a rapid decrease in the levels of adipose differentiation-related protein (ADRP). Furthermore, individual overexpression of CIDEB was sufficient to down-regulate ADRP protein levels. siRNA knockdown of CIDEB revealed that the human serum mediated increase in LD size was CIDEB-dependent. Overall, our work highlights CIDEB's role in LD fusion, and presents a new model system to study the PGC-1α/CIDEB pathway's role in LD dynamics and the VLDL pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 2, 15 November 2013, Pages 447-452
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 2, 15 November 2013, Pages 447-452
نویسندگان
Ragunath Singaravelu, Rodney K. Lyn, Prashanth Srinivasan, Julie Delcorde, Rineke H. Steenbergen, D. Lorne Tyrrell, John P. Pezacki,