کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10757350 1050394 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
βTrCP-mediated ubiquitylation regulates protein stability of Mis18β in a cell cycle-dependent manner
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
βTrCP-mediated ubiquitylation regulates protein stability of Mis18β in a cell cycle-dependent manner
چکیده انگلیسی
Ubiquitin E3 ligases including SCF complex are key regulators of cell cycle. Here, we show that Mis18β, a component of Mis18 complex governing CENP-A localization, is a new substrate of βTrCP-containing SCF complex. βTrCP interacted with Mis18β exclusively during interphase but not during mitosis and mediated proteasomal degradation of Mis18β leading to the inactivation of Mis18 complex during interphase. In addition, uncontrolled stabilization of Mis18β caused cell death. Together, we propose that βTrCP-mediated regulation of Mis18β stability is a mechanism to restrict centromere function of Mis18 complex from late mitosis to early G1 phase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 1, 3 January 2014, Pages 62-67
نویسندگان
, , , , , ,