کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10757820 1050398 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Episodic positive selection during the evolution of naphthalene dioxygenase to nitroarene dioxygenase
ترجمه فارسی عنوان
انتخاب مثبت اپیزودیک در طی تکامل نفتالین دیوکسژیناز به نیتروآرن دیوکسیاژناز
کلمات کلیدی
اکسیژناز حلقه هیدروکسیلینگ معطر، دیاکسینژاز نفتالین، دی اکسیژناز نیتروآرن، تکامل سازنده، انتخاب مثبت اپیزودیک،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Using different maximum-likelihood models of adaptive evolution, signatures of natural selective pressure, operating across the naphthalene family of dioxygenases, were examined. A lineage- and branch-site specific combined analysis revealed that purifying selection pressure dominated the evolutionary history of the enzyme family. Specifically, episodic positive Darwinian selection pressure, affecting only a few sites in a subset of lineages, was found to be responsible for the evolution of nitroarene dioxygenases (NArDO) from naphthalene dioxygenase (NDO). Site-specific analysis confirmed the absence of diversifying selection pressure at any particular site. Different sets of positively selected residues, obtained from branch-site specific analysis, were detected for the evolution of each NArDO. They were mainly located around the active site, the catalytic pocket and their adjacent regions, when mapped onto the 3D structure of the α-subunit of NDO. The present analysis enriches the current understanding of adaptive evolution and also broadens the scope for rational alteration of substrate specificity of enzyme by directed evolution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 440, Issue 1, 11 October 2013, Pages 68-75
نویسندگان
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