کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10757973 1050401 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The functional role of SUMO E3 ligase Mms21p in the maintenance of subtelomeric silencing in budding yeast
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The functional role of SUMO E3 ligase Mms21p in the maintenance of subtelomeric silencing in budding yeast
چکیده انگلیسی
In Saccharomyces cerevisiae, subtelomeric silencing is involved in the propagation of Silent Information Regulator (SIR) proteins toward euchromatin. Numerous mechanisms are involved in antagonizing the local spread of Sir-dependent silent chromatin into neighboring euchromatin. Here, we identified a novel role for sumoylation E3 ligase Mms21 in the maintenance of subtelomeric silencing. We found that disruption of E3 ligase activity of Mms21 results in the de-repression of subtelomeric silencing. Deletion of E3 ligase domain of Mms21 led to decreased binding of Sir2p, Sir3p and Sir4 at subtelomeric chromatins and increased H3K4 tri-methylation at telomere-distal euchromatin regions, correlating with increased gene expression in two subtelomeric reporter genes. In addition, a mms21Δsl mutant caused a severe growth defect in combination with htz1Δ deletion and showed an enhanced association of Htz1 with telomere proximal regions. Taken together, our findings suggest an important role of Mms21p; it contributes to subtelomeric silencing during the formation of a heterochromatin boundary.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 438, Issue 4, 6 September 2013, Pages 746-752
نویسندگان
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