کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10758058 | 1050403 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Senescent endothelial cells are prone to TNF-α-induced cell death due to expression of FAS receptor
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کلمات کلیدی
TNFNOS3eGFPLSSRT-PCRFASGAPDHHUVECSSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNALaminar shear stress - استرس برش ورقHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانEndothelial cells - سلولهای اندوتلیالtumor necrosis factor - فاکتور نکروز تومورCell death - مرگ سلولی Nitric oxide synthase 3 - نیتریک اکساید سنتاز 3Nitric oxide - نیتریک اکسیدReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استSenescence - پیریglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازFas receptor - گیرنده Fas
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The senescent endothelial cells show various phenotypes which can increase the incidence of inflammatory cardiovascular diseases, but the fundamental basis for such phenotypic changes of senescing cells remains to be elucidated. This study was undertaken to find transmembrane receptors that might be highly expressed in senescent endothelial cells and play a key role in cell death signal transduction. Comparison of mRNA expression in young and senescent human umbilical vein endothelial cells, using a cDNA microarray method, provided a list of transmembrane receptors including the FAS receptor (tumor necrosis factor receptor superfamily member 6) whose expression levels were significantly increased by cellular senescence. Additional studies focused on FAS demonstrated that a high expression of FAS receptor in senescent endothelial cells is responsible for the susceptibility to apoptotic cell death, as the siRNA-mediated suppression of FAS expression in senescent cells prevented the cell death, and overexpression of exogenous FAS in young cells increased cell death. We also verified that FAS expression level was closely associated with the activation of caspase-3 and caspase-9 involved in apoptosis. The senescence-induced transmembrane receptors including the FAS receptor may provide novel therapeutic targets to prevent cardiovascular diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 438, Issue 2, 23 August 2013, Pages 277-282
Journal: Biochemical and Biophysical Research Communications - Volume 438, Issue 2, 23 August 2013, Pages 277-282
نویسندگان
Hyeona Jeon, Yong Chool Boo,