کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10759504 | 1050425 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Estrogen-induced cell signaling in the sexually dimorphic nucleus of the rat preoptic area: Potential involvement of cofilin in actin dynamics for cell migration
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Estrogen-induced cell signaling in the sexually dimorphic nucleus of the rat preoptic area: Potential involvement of cofilin in actin dynamics for cell migration Estrogen-induced cell signaling in the sexually dimorphic nucleus of the rat preoptic area: Potential involvement of cofilin in actin dynamics for cell migration](/preview/png/10759504.png)
چکیده انگلیسی
Estrogen is a key factor to induce the sexually dimorphic nucleus (SDN) in the preoptic area (POA) of the rat brain. Identification of estrogen-dependent signaling pathways at SDN in POA during the critical period is a prerequisite for elucidating the mechanism. In the present study, we treated female rats with/without 17β-estradiol (E2) at birth, designated as postnatal day 1 (P1), and prepared total RNA from brain slices containing SDN for DNA microarray analysis. Among the estrogen-responsive genes identified, protein kinase C-delta (PKC-δ) was significantly up-regulated by E2 at P5. We examined the downstream effectors of PKC-δ protein by Western blotting and found an E2-induced PKC-δ/Rac1/PAK1/LIMK1/cofilin pathway. In the pathway, E2 suppressed the phosphorylation (inactive form) of cofilin. This result was supported by immunohistochemistry, where the phosphorylation/dephosphorylation of cofilin occurred at SDN, which suggests that cell migration is a cue to create sexual dimorphism in POA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 434, Issue 2, 3 May 2013, Pages 287-292
Journal: Biochemical and Biophysical Research Communications - Volume 434, Issue 2, 3 May 2013, Pages 287-292
نویسندگان
Yuko Wada-Kiyama, Chiaki Suzuki, Tomohiro Hamada, Dilip Rai, Ryoiti Kiyama, Makoto Kaneda, Yasuo Sakuma,