Reconstitution of functionally efficient SecA-dependent protein-conducting channels: Transformation of low-affinity SecA-liposome channels to high-affinity SecA-SecYEG-SecDF·YajC channels

► SecA-liposomes alone are functional as protein-conducting channels. ► SecYEG increases efficiency and gains signal peptide specificity. ► SecDF·YajC enhances efficiency of the SecA-SecYEG-liposomes. ► SecA-liposomes with SecYEG/DF·YajC functions as protein-conducting channels.