Insulin suppression of apolipoprotein B in McArdle RH7777 cells involves increased sortilin 1 interaction and lysosomal targeting

► Insulin suppresses VLDL B100 secretion via PI3K in McArdle RH7777 rat hepatoma cells. ► Insulin enhances sortilin 1-B100 interaction. ► Inhibition of lysosomal degradation by BafA1 blocks insulin suppression of VLDL B100. ► BafA1 inhibition reveals increased insulin-dependent sortilin 1-B100 interaction. ► Sortilin 1-B100 interaction likely mediates insulin-dependent B100 degradation.