Correlation between cell attachment areas after 2Â h of culture and osteogenic differentiation activity of rat mesenchymal stem cells on hydroxyapatite substrates with various surface properties

► Small MSCs show high osteogenic differentiation activity. ► Large MSCs show low osteogenic differentiation activity. ► Osteogenic differentiation well correlates with cell attachment areas of MSCs. ► Later osteogenic differentiation can be estimated by cell attachment areas of MSCs.