27-Hydroxycholesterol induces production of tumor necrosis factor-alpha from macrophages

► 27OHChol or 7αOHChol induced transcription and intracellular expression of TNF-α in macrophages. ► Exposure of 27OHChol- or 7αOHChol-treated THP-1 macrophages to LPS resulted in augmentation in TNF-α production. ► TO-901317, pertussis toxin, PP2, and LY294002 attenuated expression of TNF-α induced by oxysterols.