Prolactin stimulates the L-type calcium channel-mediated transepithelial calcium transport in the duodenum of male rats

► PRL enhanced the transcellular and paracellular duodenal Ca2+ transport in male rats. ► The PRL-enhanced transcellular Ca2+ transport was dependent on L-type Ca2+ channel. ► Both PMCA and NCX1 were essential for the basolateral Ca2+ efflux. ► JAK2, MEK, PI3K and Src mediated PRL signaling in the duodenal cells of male rats.