Acid-activated prorenin binds to (pro)renin receptor in vitro

► The renin activity of acidified prorenin (free form) depends on time and pH. ► Acidified prorenin at pH 4.5, 5.5, and 6.5 has similar binding affinity to (P)RR. ► Acidified prorenin at pH 3.3 hardly binds to (P)RR. ► (P)RR-bound acidified prorenin also shows the renin activity. ► Acidified prorenin could lead to pathogenesis of diseases by modulating RA system.