Tandem zyxin LIM sequences do not enhance force sensitive accumulation

► We hypothesize that multiple zyxin LIM domains may enhance force sensitivity. ► We analyze the accumulation of tandem dimer or trimer LIM domain and traction force. ► Tandem LIM variants reduces VASP accumulation at focal adhesions. ► Force sensitivities are similar among single and tandem LIM variants. ► Single LIM domain of zyxin has the optimal force sensitivity.