Differential immunoreactivity of goat derived scrapie following in vitro misfolding versus mouse bioassay

► Tg338 mouse brain supports prion misfolding by PMCA when seeded with caprine brain. ► Antibody detection varies between PrPres resulting from PMCA versus mouse bioassay. ► The N-terminal P4 epitope is more readily maintained following PMCA versus bioassay.