Identification of ryanodine receptor isoforms in prostate DU-145, LNCaP, and PWR-1E cells

► Prostate epithelial cells respond to caffeine, a ryanodine receptor activator. ► Tumor lines DU-145 expressed RyR type 1, LNCaP only expressed RyR2. ► Non-tumor prostate epithelial PWR-1E cells expressed RyR types 1 and 2. ► Non-excitatory cells have RyRs, but isoform does not predict tumorigenicity.