The binding mechanism of eIF2β with its partner proteins, eIF5 and eIF2Bε

► We reconstructed (eIF5-CTD)-(eIF2β-NTD) and (eIF2Bε-CTD)-(eIF2β-NTD) in vitro. ► We examined structural changes of eIF2β-NTD, eIF5-CTD and eIF2Bε-CTD. ► We found that eIF2β-NTD works as an intrinsically disordered domain. ► We propose the binding mechanism of eIF2β-NTD with eIF5-CTD and eIF2Bε-CTD.