PKC-ε mediates multiple endothelin-1 actions on systolic Ca2+ and contractility in ventricular myocytes

► Dn-PKC-ε and dn-PKC-δ showed distinct subcellular localization in adult myocytes. ► ET-1 induces positive inotropy, increase in [Ca2+]i and increase in Ca sensitivity. ► The effects of ET-1 were abolished by dn-PKC-ε overexpression but not by dn-PKC-δ. ► The data suggest that PKCε has a critical role in ET-1-induced changes.