Accumulation of pre-let-7g and downregulation of mature let-7g with the depletion of EWS

► The depletion of EWS resulted in the accumulation of precursor let-7g but down-regulates mature let-7g in U2OS cells. ► Mature let 7g was suppressed in both Ewing sarcoma cells and primary Ewing sarcoma. ► Understanding how EWS mediates miRNA expression may provide invaluable information for therapeutic targets in Ewing sarcoma.