PipB2 is a substrate of the Salmonella pathogenicity island 1-encoded type III secretion system

► CyaA-based screens are used to detect Salmonella type III secretion system effectors. ► PipB2, a T3SS2 effector, is also translocated to host cells through T3SS1. ► T3SS1-dependent translocation of PipB2 is detected in several mammalian cell types. ► PipB2 translocation needs the N-terminal 10 amino acid residues.