LPS activates ADAM9 dependent shedding of ACE from endothelial cells

► LPS induces ACE shedding from endothelial cells by a metalloproteinase. ► LPS induced ACE shedding requires NF-κB, protein synthesis, TNFα and TNFRI/II. ► The LPS activated ACE secretase is ADAM9. ► Soluble TNFα does not activate ADAM9 dependent shedding of ACE. ► Phorbol ester (PMA) does not induce ACE shedding in endothelial cells.