About and beyond the Henri-Michaelis-Menten rate equation for single-substrate enzyme kinetics

► Critique of recent evaluation and generalization of Henri-Michaelis-Menten equation. ► Numerical simulations show recent generalized formula to be a poor approximation. ► New generalized rate expression for simple enzyme reaction mathematically derived. ► Reaction rate error calculations show adequacy of new reaction rate formula. ► New generalized rate equation is acceptable approximation even when E0 in excess.