Coordinated regulation of IL-4 and IL-13 expression in human T cells: 3C analysis for DNA looping

► In human TH2 cells, IL4 and IL13 genes are regulated in a coordinated manner. ► IL4 and IL13 are both regulated by the transcription factors NFAT1 and TBET. ► Upon cell stimulation, NFAT1 translocates into the nucleus. ► In the nucleus NFAT1 associates with GATA3 at the promoter regions of IL4 and IL13. ► 3C analysis showed looping of IL4 and 13 to form a structure poised for transcription.