The AAA-ATPase NVL2 is a telomerase component essential for holoenzyme assembly

► Identification of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. ► NVL2 associates with catalytically active telomerase via an interaction with hTERT. ► NVL2 is a telomerase component essential for holoenzyme assembly. ► ATP-binding activity of NVL2 is required for hTERT binding and telomerase assembly.