In vitro glycation of human serum albumin by dihydroxyacetone and dihydroxyacetone phosphate

► Dihydroxyacetone phosphate and dihydroxyacetone are potent glycation agents. ► Glycation of serum albumin is pH, temperature and sugar concentration dependent. ► Dihydroxyacetone is more reactive than dihydroxyacetone phosphate.