Embryonic stem cell differentiation system for evaluating gene functions involved in physiological megakaryocytic differentiation

► We generated a system to study gene function in normal megakaryocytopoiesis. ► ES cells with transgenes differentiate into megakaryocytes and proplatelets. ► Transgene expression in the Hprt locus is suppressed in megakaryocytic cells. ► β-Globin insulators drastically improve transgene expression. ► Transgene is activated specifically in megakaryocytic cells.