Transgene insertion in intronic sequences of Mdga2 gene shows methylation in an imprinted manner in an Acrodysplasia (Adp) mouse line

► The Acrodysplasia (Adp) mutation arose from the transgene insertion. ► Precise imprinting mechanisms and transgene integration sites are not proven in Adp. ► The transgene was inserted into the intronic sequences of the Mdga2 gene. ► The transgene shows methylation in an imprinted manner in Adp. ► Methylation of the transgene seems to relate to the phenotypic expression of Adp.