Membrane topology of murine glycerol-3-phosphate acyltransferase 2

► GPAT2 protein has two transmembrane segments. ► The N-terminus and the C-terminus of the GPAT2 protein are exposed to the cytosol. ► The enzymatically active motifs I, II and III of the GPAT2 protein face the cytosol. ► The active motif IV of the GPAT2 protein is located within the organelle membrane.