Characterization of the ptr5+ gene involved in nuclear mRNA export in fission yeast

► We cloned the ptr5+ gene involved in nuclear mRNA export in fission yeast. ► The ptr5+ gene was found to encode nucleoporin 85 (Nup85). ► Seh1p and Mlo3p are multi-copy suppressors for the ptr5 mutation. ► Ptr5p/Nup85p functions in nuclear mRNA export through the mRNA export factor Rae1p. ► Ptr5p/Nup85p interacts genetically with pre-mRNA splicing factors.