Activated T-cells and pro-inflammatory cytokines differentially regulate prostaglandin E2 secretion by mesenchymal stem cells

► BM- and Ad-MSCs produce high amounts of PGE2 in the presence of activated T-cells. ► The elicitation of the PGE2 secretion of MSCs is contact-dependent. ► TNF-α and IFN-γ act synergistically to induce PGE2 production. ► L-NMA could only partially inhibit PGE2 secretion in response to TNF-α and IFN-γ. ► The PGE2 secretion of MSCs is not mediated by a unique molecular mechanism.