Expression, purification, and characterization of a 2,3-dihydroxybiphenyl-1,2-dioxygenase from Bacillus sp. JF8 in Escherichia coli

► Synthesized the BsbphCI gene encoding a 2,3-DHBP dioxygenase. ► Purified recombinant 2,3-DHBP dioxygenase. ► Determined properties of the recombinant enzyme. ► The transgenic strain exhibited a strong capacity of degradation.