NeuroD1 is an upstream regulator of NSCL1

► NeuroD1 and NeuroD2 activate NSCL1 expression in vitro. ► NeuroD1 is required for NSCL1 expression in the cerebellum. ► NSCL1 does not require NeuroD2 for transcription in the cerebellum. ► NSCL2 does not require NeuroD1 or NeuroD2 for expression.