Role of lipid rafts in porcine reproductive and respiratory syndrome virus infection in MARC-145 cells

► Cellular membrane and viral cholesterol are important for the PRRSV infection on MARC-145 cells. ► Cholesterol depletion affects PRRSV entry. ► Cholesterol depletion did not change expression levels of CD163 in MARC-145 cells. ► Cholesterol depletion disturbs lipid-raft-dependent endocytosis.