Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans

► Gox2181 was active towards a wide range of substrates with NAD(H) as a cofactor. ► Gox2181 displayed preference for secondary hydroxyl or carbonyl groups. ► X-ray crystal structure of Gox2181 was determined to a resolution of 1.8 Å. ► Gox2181 was denoted SDR51C based on the SDR nomenclature system. ► The biochemical and structural analysis of Gox2181 enriched the SDR superfamily.