In vitro prominent bone regeneration by release zinc ion from Zn-modified implant

► We isolated the Zn2+ ions (eluted Zn2+ ion; EZ) from zinc-incorporated titanium implant. ► The EZ promoted the cell viability in hBMCs. ► The EZ stimulated preosteoblast and osteoblast marker gene expression in hBMCs. ► The hBMCs supplemented with EZ showed typically cell morphology when osteoblast maturing. ► It is revealed that the EZ also stimulates the calcium deposition of hBMCs.