Proteomic analysis of cancer stem cells in human prostate cancer cells

► DU145 prostate cancer cell line was isolated into CD44+ or CD44− cells. ► We confirmed CD44+ DU145 cells are more proliferative and tumorigenic than CD44− DU145 cells. ► We analyzed and identified proteins that were differentially expressed between CD44+ and CD44− DU145 cells. ► Cofilin and Annexin A5 associated with cancer were found to be positively correlated with CD44 expression.