Characterization of an AtCCX5 gene from Arabidopsis thaliana that involves in high-affinity K+ uptake and Na+ transport in yeast

► The AtCCX5 protein coding a putative cation calcium exchanger was characterized. ► AtCCX5 expressed in yeast was localized in the plasma membrane and nuclear periphery. ► AtCCX5 protein did not show the same transport properties as the CAXs. ► AtCCX5 protein involves in mediating high-affinity K+ uptake in yeast. ► AtCCX5 protein also involves in Na+ transport in yeast.