Plural assay systems derived from different cell lines and hepatitis C virus strains are required for the objective evaluation of anti-hepatitis C virus reagents

► We recently developed a new cell line-derived HCV drug assay system. ► We recently developed a new HCV strain-derived HCV drug assay system. ► We evaluated 26 anti-HCV reagents reported to date using these assay systems. ► Anti-HCV activities of reagents differed among the used cell lines or HCV strains. ► Objective evaluation of anti-HCV reagents needs plural assay systems.