Sialyl Lewisx-dependent binding of human monocyte-derived dendritic cells to selectins

► The role of the selectin binding determinant sLex was studied in mo-DCs. ► sLex is required for maximal mo-DC binding to endothelial cells. ► Mo-DCs bind to selectins via a sLex-dependent pathway. ► PSGL-1 is indispensable for P- and L-, but not E-selectin recognition. ► IFN-γ increases sLex expression in mo-DCs, probably by enhancing C2GnT-I synthesis.