A cleavable signal peptide enhances cell surface delivery and heterodimerization of Cerulean-tagged angiotensin II AT1 and bradykinin B2 receptor

► A new FRET-based method detects AT1/B2 receptor heterodimerization. ► First time application of AT1-Cerulean as a FRET donor. ► Method relies on signal peptide-enhanced cell surface delivery of AT1-Cerulean. ► A high FRET efficiency revealed efficient heterodimerization of AT1/B2R proteins. ► AT1/B2R heterodimers were functionally coupled to desensitization mechanisms.