Analysis of apolipoprotein A-I as a substrate for matrix metalloproteinase-14

► MMP-14 degrades apoA-I more efficiently than other tested MMPs. ► Lipid-free apoA-I is more susceptible to MMPs than lipid-bound apoA-I. ► MMP-14 cleavage sites on apoA-I have been determined. ► Cleavage of apoA-I by MMP-14 impairs its ability to form HDL.