Human HOXA5 homeodomain enhances protein transduction and its application to vascular inflammation

► We have developed an E. coli protein expression vector including human specific gene sequences for protein cellular delivery. ► The plasmid was generated by ligation the nucleotides 770-817 of the homeobox A5 mRNA sequence. ► HOXA5-APE1/Ref-1 inhibited TNF-alpha-induced monocyte adhesion to endothelial cells. ► Human HOXA5-PTD vector provides a powerful research tools for uncovering cellular functions of proteins or for the generation of human PTD-containing proteins.