Apical localization of PMCA2w/b is enhanced in terminally polarized MDCK cells

► MDCK cell clones stably expressing PMCA2w/b were characterized. ► PMCA2w/b in MDCK cells efficiently removes Ca2+ following a transient Ca2+ spike. ► Apical localization of PMCA2w/b is enhanced in fully polarized, filter-grown MDCK cells. ► Endogenous NHERF2 promotes apical enrichment of PMCA2w/b via the membrane cytoskeleton.