Yellow fever virus NS2B/NS3 protease: Hydrolytic Properties and Substrate Specificity

► YFV contains the same substrate motif in all processing sites. ► The kcat/km varied by more than two orders of magnitude among then. ► Obtained data parallel with those previously obtained in vivo. ► Substrates do not co-evolved with protease to promote highly efficient hydrolysis. ► Interactions others than on motif are critical in mediating substrate selective.