Protein kinase C βII and δ/θ play critical roles in bone morphogenic protein-4-stimulated osteoblastic differentiation of MC3T3-E1 cells

► BMP-4 promotes osteocalcin synthesis in osteoblast-like cells. ► PKC plays an important role in BMP-4-mediated osteocalcin synthesis. ► PKC βII and PKC δ/θ regulates osteoblastic differentiation and mineralization. ► PKCs are involved in BMP-4-mediated VEGF production.