Inhibitory effect of leptin on rosiglitazone-induced differentiation of primary adipocytes prepared from TallyHO/Jng mice

► In this study, we investigated the effects of leptin on adipocyte differentiation prepared from subcutaneous fat of TallyHo mice. ► Leptin inhibited the adipocytes differentiation at physiological concentration via inhibition of PPARγ expression. ► Inhibitors of ERK and STAT1 restored the leptin's inhibitory activity both in vitro and in vivo.