Induction of an osteocyte-like phenotype by fibroblast growth factor-2

► FGF2 induces an osteocyte-like phenotype in osteoblasts and stromal cells. ► FGF2 dramatically increases gene expression of DMP1. ► FGF2 suppression of SOST may contribute to the osteoanabolic effect of FGF2 in vivo. ► DMP1 and E11 expression precedes the formation of osteocyte-like cell processes. ► Model to study osteocyte biology to complement cell lines, such as MLO-Y4 cells.